NEW METHOD FOR EXPERIMENTAL MODELING OF PANCREAS TRAUMAS FOR WISTAR RATS AND CYTOKINE LEVEL CHANGES IN THESE MODELS

Edgars Zarembo (), Olga Mežale (), Aleksandrs Mikitins (), Leontine Antonovica (), Jekaterina Petla () and Aigars Petersons ()
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Edgars Zarembo: Riga Stradins University
Olga Mežale: Riga Stradins University
Aleksandrs Mikitins: Riga Stradins University
Leontine Antonovica: Riga Stradins University
Jekaterina Petla: Riga Stradins University
Aigars Petersons: Riga Stradins University

CBU International Conference Proceedings, 2013, vol. 1, issue 0, 254-257

Abstract: The incidence of pancreas traumas (PT) is low; however, lethality in case of PT remains unchangingly high. The aim of the present study is to develop a technically simple, easily repeatable method for experimental modelling of PT of various degrees of severity for Wistar rats and study changes in plasma levels of cytokines (IL-1β; IL-6; TNFα; IL-10; MCP-1; IL-12(p70)) for experimental models. Under general anaesthesia 50 animals were performed laparotomy and were made damages of pancreas tissues of various severity standardized using Crile clamps. 24 h and 72 h after creating PT the samples of blood serum were taken.In autopsy all animals were identified the features of pancreatitis. Severity of pancreatic histologic injury was associated with the impact severity. Plasma MCP-1 levels were significantly (p<0,05) increased in 24 h and 72 h samples. The method with putting Crile clamps under general anaesthesia shall be applied for creation of PT for Wistar rats and let model various degrees of PT severity. MCP-1 might play a pivotal role in inflammatoryresponse of acute pancreatitis.

Keywords: pancreas; trauma; pancreatitis; model; cytokines (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:aad:iseicj:v:1:y:2013:i:0:p:254-257

DOI: 10.12955/cbup.v1.41

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