HIGHER CTX-M, TEM, AND SHV EXTENDED-SPECTRUM BETA-LACTAMASE PLASMID GENE COMBINATION FREQUENCY IN ESBL PRODUCING KLEBSIELLA PNEUMONIAE COMPARED WITH ESBL PRODUCING ESCHERICHIA COLI

Vita Skuja (), Katrīna Pekarska (), Aleksejs Derovs (), Ludmila Vīksna (), Linda Piekuse (), Inga Kempa (), Una Caune (), Dace Rudzīte (), Aivars Lejnieks () and Angelika Krūmiņa ()
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Vita Skuja: Riga Stradins University
Katrīna Pekarska: Riga Stradins University
Aleksejs Derovs: Riga Stradins University
Ludmila Vīksna: Riga Stradins University
Linda Piekuse: Riga Stradins University
Inga Kempa: Riga Stradins University
Una Caune: Riga Stradins University
Dace Rudzīte: Riga Stradins University
Aivars Lejnieks: Riga Stradins University
Angelika Krūmiņa: Riga Stradins University

CBU International Conference Proceedings, 2016, vol. 4, issue 0, 732-739

Abstract: Introduction:Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae confer antibiotic resistance to broad-spectrum penicillins, cephalosporins, using ESBL genes CTX-M, TEM, SHV, which are encoded in bacterial plasmid genome. Methods: We compared plasmid CTX-M, TEM, and SHV ESBL gene combinations in ESBL producing Escherichia coliand Klebsiella pneumoniae. Altogether, 136 ESBL producing Enterobacteriaceae isolation cases were analyzed. Results:The ESBL producing Enterobacteriaceae, mostly K. pneumoniae (n = 66; 48.53%), E. coli (n = 36; 26.47%), were isolated from 52 (38.2%) female and 84 (61.8%) male patients from Riga East Clinical University Hospital. Overall,132 isolates (97.06%) registered positive for the plasmid CTX-M gene, 97 isolates (71.32%) for the plasmid TEM gene, and 87 isolates (63.97%) for the plasmid SHV gene.Patients with the three ESBL plasmid gene (CTX-M+ TEM+ SHV+) combination were significantly older (67.11 ± 14.16 years) than patients with other gene combinations (59.63 ± 18.14 years; p = 0.047). Plasmid SHV gene frequency was higher in K. pneumoniae (p < 0.001). The K. pneumoniae mostly presented with the three plasmid ESBL gene (CTX-M+ TEM+ SHV+) combination, whereas E. coli presented with other gene combinations (p = 0.014). Conclusions: Klebsiella Pneumoniae, more often, presented with the plasmid SHV ESBL gene and the three ESBL gene (CTX-M+ TEM+ SHV+) combination, compared to E. coli. Older patients with ESBL producing Enterobacteriaceae infection, more often, presented with the three ESBL gene (CTX-M+ TEM+ SHV+) combination, compared to younger patients with the infection.

Keywords: Extended-spectrum beta-lactamaseCTX-M; TEM; SHV; Klebsiella pneumoniae; Escherichia coli (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:aad:iseicj:v:4:y:2016:i:0:p:732-739

DOI: 10.12955/cbup.v4.841

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