APPLICATION OF SOMATIC EMBRYOGENESIS AND BIOREACTOR TECHNIQUES IN MICROPROPAGATION OF TROPICAL LILY CONCA D’OR (ORIENT X TRUMPET)

Nguyen Thanh Quang () and Tran Van Minh ()
Additional contact information
Nguyen Thanh Quang: International University, Vietnam National University Ho Chi Minh City, Viet Nam
Tran Van Minh: International University, Vietnam National University Ho Chi Minh City, Viet Nam

CBU International Conference Proceedings, 2017, vol. 5, issue 0, 1247-1252

Abstract: The lily is considered one of the highest value cutting flowers as well as is popular to the majority. Bulbs are often imported from the Netherland with an expensive price. Thus, it is difficult to sell commercial lily flowers in the domestic market and international trade. This study focused on mass producing the source of somatic embryos with a high regeneration ability used for the lily micropropagation. Internodes were cultured on MS medium and supplemented with 0.1 mg/l BA and 0.5 mg/l NAA to induce callus, while embryogenic cells reached the highest value at 98.2% before such embryogenic callus was then multiplied on the same media within 5 weeks. The suitable media for somatic embryos regeneration was MS medium supplemented with 0.5 mg/l BA and 0.25 mg/l NAA which after 5 weeks of culture gave us a shoot number of 3.7 shoots/clusters, a shoot height of 87.3 mm, a leaf diameter of 7.7 mm. Multiple shoots regenerated from embryogenic cells were then used as materials for micropropagation. Results showed that the highest data for multiple shoot multiplication was reached in the bioreactor culture system with the maximum number of shoot height (100.25 mm), shoot number (7.1 shoot/cluster) and fresh weight of shoots (1470.94 mg/cluster). The suitable medium for rooting was MS medium supplemented with 0.5 mg/l NAA.

Keywords: Conca D’orbulb; somatic embryo; induction; proliferation; regeneration (search for similar items in EconPapers)
Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://ojs.journals.cz/index.php/CBUIC/article/view/1104/1491 (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:aad:iseicj:v:5:y:2017:i:0:p:1247-1252

DOI: 10.12955/cbup.v5.1104

Access Statistics for this article

More articles in CBU International Conference Proceedings from ISE Research Institute
Bibliographic data for series maintained by Petr Hájek ().