RAPD-ANALYSIS OF CYCLAMEN SPP. GENOME POLYMORPHISM

Elza Makaradze (), Galina Meparishvili (), Natela Varshanidze (), Inga Diasamidze (), Ketevan Dolidze (), Eteri Jakeli () and Nana Zarnadze ()
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Elza Makaradze: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi
Galina Meparishvili: Batumi Shota Rustaveli State University, Institute of Phytopathology and Biodiversity, Kobuleti
Natela Varshanidze: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi
Inga Diasamidze: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi
Ketevan Dolidze: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi
Eteri Jakeli: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Pharmacy, Batumi
Nana Zarnadze: Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi

CBU International Conference Proceedings, 2019, vol. 7, issue 0, 949-953

Abstract: Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

Keywords: Cyclamen adzharicum; Cyclamen coum; RAPD-PCR (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:aad:iseicj:v:7:y:2019:i:0:p:949-953

DOI: 10.12955/cbup.v7.1483

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