Calcium Buffering by Fluorescent Indicators – Implications and Easy Solutions

Krzysztof L Hyrc
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Krzysztof L Hyrc: The Hope Center for Neurological Disorders, Alafi Neuroimaging Laboratory and Department of Neurology, Washington University School of Medicine, USA

Biomedical Journal of Scientific & Technical Research, 2023, vol. 53, issue 3, 44687-44703

Abstract: The determination of cytosolic free calcium concentration ([Ca2+]i ) an important secondary messenger [1-4], is typically accomplished with the use of fluorescent indicators [5-8]. Varying widely with respect to their spectral and binding properties [9-12], indicators share common design and operation principles. A typical indicator molecule comprises a calcium binding moiety, usually BAPTA [13] or a derivative [9], linked to a fluorophore, such as stilbene [14], fluorescein or rhodamine [15].

Keywords: Journals on Medical Drug and Therapeutics; Journals on Emergency Medicine; Physical Medicine and Rehabilitation; Journals on Infectious Diseases Addiction Science and Clinical Pathology; Open Access Clinical and Medical Journal; Journals on Biomedical Science; List of Open Access Medical Journal; Journals on Biomedical Engineering; Open Access Medical Journal; Biomedical Science Articles; Journal of Scientific and Technical Research (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:abf:journl:v:53:y:2023:i:3:p:44687-44703

DOI: 10.26717/BJSTR.2023.53.008398

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