Methodological Approaches to Contrasting Sections for Electron Microscopy

Maksimovich NYe, Bon Ei, Ostrovskaya Ob and Kurochkina Ed
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Kurochkina Ed: Grodno State Medical University, Republic of Belarus

Biomedical Journal of Scientific & Technical Research, 2024, vol. 55, issue 2, 46921-46924

Abstract: Electron microscopy is the leading method for studying the ultrastructure of cells. The main method of preparing tissue for research is contrast. Tissue sections, even fixed in solutions of O3 O4 or KMnO4 , i.e. in solutions that simultaneously serve as both fixative and contrasting agent, are usually insufficiently contrasted. Greater contrast can be achieved by selectively increasing the density of the structures. High molecular weight contrast agents, preferably heavy metals and their compounds, are suitable for this purpose.

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Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:abf:journl:v:55:y:2024:i:2:p:46921-46924

DOI: 10.26717/BJSTR.2024.55.008690

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