A Rapid Fluorescent In Vitro Assay Suitable for Studying the Kinetics of O6-Alkylguanine Lesion Progression to DNA Inter-Strand Cross-Links and the Kinetics of the Primary Lesion’s Repair

Philip G Penketh
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Philip G Penketh: Department of Pharmacology, Yale University School of Medicine, USA

Biomedical Journal of Scientific & Technical Research, 2024, vol. 59, issue 3, 51659-51661

Abstract: In the author’s opinion, by far the best assay for MGMT activi- ty in cells is the assay developed by Ishiguro, et al. [1-3]. This assay is based on the transfer of radioactive benzyl residues from [ben- zene-3H]O6-benzylguanine to MGMT However, this assay is unsuitable for studying the kinetics of the progression of the primary O6-alkyl- guanine lesion to DNA-DNA inter-strand cross-links, and the kinetics of the primary lesion’s repair by MGMT, because the rate constants involved in the Ishiguro assay means it takes several hours to deter- mine the MGMT activity, whereas many of the dynamic processes we are interested in occur on the time frames of minutes or even seconds.

Keywords: Journals on Medical Drug and Therapeutics; Journals on Emergency Medicine; Physical Medicine and Rehabilitation; Journals on Infectious Diseases Addiction Science and Clinical Pathology; Open Access Clinical and Medical Journal; Journals on Biomedical Science; List of Open Access Medical Journal; Journals on Biomedical Engineering; Open Access Medical Journal; Biomedical Science Articles; Journal of Scientific and Technical Research (search for similar items in EconPapers)
Date: 2024
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DOI: 10.26717/BJSTR.2024.59.009314

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