Selection of Enterobacter cloacae Strain POPE6 for Fermentative Production of Extracellular Lipase on Palm Kernel Oil Processing Effluent

Atim Iboyo, Atim David Asitok, Maurice George Ekpenyong and Sylvester Peter Antai

International Journal of Sciences, 2017, vol. 6, issue 11, 1-17

Abstract: An Enterobacter cloacae strain was isolated from palm oil processing effluent and demonstrated ability to elaborate extracellular lipase on Tween 80-minimal medium using the rapid plate assay of precipitate formation. Peak productivity of the enzyme in submerged culture occurred at 72 h with lipase concentration of 95 μg/mL. Enzyme activity, monitored using different carbon-chain lengths of p-nitrophenol (p-NP)-triacylglycerol substrates, revealed that stearate was the most suitable substrate for the lipase. Preliminary optimization of major medium variables revealed highest lipase concentration in palm kernel oil processing effluent (Carbon source; P = 2.22E-05; Time, P = 1.99E-25; P

Date: 2017
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DOI: 10.18483/ijSci.1482

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