Effects of Endogenous n-3PUFA on Body Weight, Autophagy and Inflammation in Mice

Xin Fang, Chao Song, YinLin Ge and JinYu Zhang

International Journal of Sciences, 2018, vol. 7, issue 03, 64-70

Abstract: The fat-1 transgenic mice were used as models, to investigate the effects of n-3PUFAs on body weight, the expression of inflammation and autophagy in mice, and to explore its mechanism. The mice were divided into two groups: fat-1 transgenic mice and wild-type mice. The body weight and body length were measured and the index of body weight/body length was calculated regularly every week for 8 weeks. Cardiac blood was collected for determination of TG, CT, HDL-C, LDL-C and BG. Frozen sections of liver were stained by Oil Red O to observe the lipid droplets. The expression of autophagy proteins P62, LC3 and ATG7 in the hypothalamus were detected by western blot, and the relative quantitative analysis was performed. Real-time quantitative PCR was used to quantify the mRNA expression of TNF-α, IL-6, IL-1β, IFN-γ, MCP-1, TLR-4 and adiponectin in epididymal adipose tissue. The body weight/body length of fat-1 transgenic mice was significantly lower than that of wild-type mice(P

Keywords: Hypothalamus; n-3 PUFAs; Autophagy; Inflammation (search for similar items in EconPapers)
Date: 2018
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DOI: 10.18483/ijSci.1587

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