Inhibitory Effect of Phytic Acid on LPS Induced BV-2 Microglia Activation

Jinhui Zuo, Fuping Guan, Xin Li, Xiuli Wang, Fengyu Pan, Zheng Zhang and Chao Song

International Journal of Sciences, 2019, vol. 8, issue 03, 8-11

Abstract: Hyperactivation of microglia is the important pathology of neurodegenerative diseases. Being naturally occurring compound, phytic acid (PA) has been found to exert anti-inflammatory effect in MPTP-induced parkinson’s disease model of mice, but mechanism needed to be further investigated. In our study, BV-2 microglia were cultured and induced by lipopolysaccharide (LPS) to mimic inflammation models to testify the phytic acid’s effect on neuroinflammation. Methods: BV-2 microglia were cultured in vitro. After pretreatment with different doses of PA, lipopolysaccharide (LPS) was added to induce the activation of BV-2 microglia. The cell survival rate was detected by MTT colorimetry, the NO release of microglia was measured by Griess assay, and the concentration of IL-6 in supernatant of cell culture was detected by ELISA assay. DAPI staining was used to detect the changes of apoptosis of activated BV-2 microglia before and after phytic acid treatment. Results: Phytic acid could inhibit the production of NO and IL-6 in BV-2 microglia activated by LPS, and block the excessive activation of BV-2 microglia stimulated by LPS. At the same time, it can promote the apoptosis of over-activated microglia. Therefore, phytic acid may play a potential role on prevention and treatment of neurodegenerative diseases associated with its inhibition on microglial activation.

Keywords: Phytic Acid; Lipopolysaccharide; Microglia; Neuroinflammation (search for similar items in EconPapers)
Date: 2019
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DOI: 10.18483/ijSci.1901

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