Three-Dimensional Culture Model of Tumoral Cells

Palma Maria Belen, Martinez Marina, Luzzani Carlos, Andrini Laura, Errecalde , Lía, Inda Ana Maria, Pelinski P, Miriuka Santiago Gabriel and García Marcela Nilda
Additional contact information
Luzzani Carlos: University of Medicine, Argentina
Inda Ana Maria: LIAN laboratory, Argentina
García Marcela Nilda: Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina

International Journal of Cell Science & Molecular Biology, 2018, vol. 4, issue 1, 21-24

Abstract: The mammospheres are a multicellular tumor spheroid model. These are a widely used culture system to mimic the three-dimensionality of tumors. In this manuscript, we narrate the developmentof a model using a murine mammary adenocarcinoma, which we used to study tumor physiology and therapeutic applications. We test three techniques published in previous works. The best condition was used V-shaped 96-well plates with an agarose monolayer, with this we were obtained compact and robust spheroids. After that, we studied the interaction between tumoral cells and mesenchymal stem cells (MSC) derived to human umbilical cord in three dimensional culture.

Keywords: juniper publishers; Journal of Cell Science; Molecular Biology; International Journal of Cell Science; International Journal of Cell Science & Molecular Biology; molecular biology journals list; molecular biology impact factor; molecular biology scholarly journal; molecular biology online journals; molecular biology research papers; scientific journals; juniper publishers review (search for similar items in EconPapers)
Date: 2018
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:adp:ijcsmb:v:4:y:2018:i:1:p:21-24

DOI: 10.19080/IJCSMB.2018.03.555630

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