Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines

Anamika Thakur, Sanju Kumari, Utkarshini Sharma, Rohit Krishna, Kanak Sinha and Santosh Kumar
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Santosh Kumar: Department of Botany, B R A Bihar University, India

International Journal of Cell Science & Molecular Biology, 2018, vol. 5, issue 1, 49-56

Abstract: Proteases are highly required where proteins need some sort of alteration either structurally or functionally. They are widely used in household laundry, paper and leather industries as well as in several pharmaceutical compounds. Thus, the present study was confined to screen more than fifty isolates of microbes from the rhizospheres of fruit orchard for proteolytic activity on skimmed milk agar medium. Isolates SK701 and SK709 were found producing halo regions around the streaked mycelia. However, only isolate SK709 displayed maximum 64.6 U/ml protease activities in starch casein broth after 96 hours of culture at optimum conditions of 35°C and pH 7.6. It was identified as a gram-negative bacterium on the basis of staining, morphological, cultural, biochemical and physiological characteristics.

Keywords: juniper publishers; Journal of Cell Science; Molecular Biology; International Journal of Cell Science; International Journal of Cell Science & Molecular Biology; molecular biology journals list; molecular biology impact factor; molecular biology scholarly journal; molecular biology online journals; molecular biology research papers; scientific journals; juniper publishers review (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:adp:ijcsmb:v:5:y:2018:i:1:p:49-56

DOI: 10.19080/IJCSMB.2018.04.555660

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