Studies on Crossability and Genetic Diversity in Cowpea (Vigna unguiculata L. Walp.)

B Nameirakpam and Khanna Vk
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Khanna Vk: School of Crop Improvement, College of Post-Graduate Studies, Central Agricultural University, India

International Journal of Environmental Sciences & Natural Resources, 2018, vol. 13, issue 1, 08-16

Abstract: Characterization of genetic diversity among cultivated cowpea (Vigna unguiculata) varieties using RAPD markers and assessment of crossability within the species was taken up. Three parameter i.e. pollen fertility, pollen germinability and pollen tube growth rate were measured on both selfing and crossing to assess the crossability. Pollen fertility exhibited less significant correlation with fruit set and high significant correlation was found in between the pollen germinability and pollen tube growth rate with the fruit set. In both selfing and crossing, pollen germination and pollen tube growth increased in a constant rate. The crossability level was found to be better on selfing when compared with crosses. A total of 30 RAPD primers were randomly selected to assess genetic diversity of 36 accessions of cowpea. Based on the PIC value, five primers (OPC 14, OPB 1, OPA 10, OPG 13 and OPA 4) were found to be more informative. The PIC value showed a ranged from 0.597 to 0.885 with the primer OPC 14 having the highest PIC value of 0.885.

Keywords: earth and environment journals; environment journals; open access environment journals; peer reviewed environmental journals; open access; juniper publishers; ournal of Environmental Sciences; juniper publishers journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:adp:ijesnr:v:13:y:2018:i:1:p:08-16

DOI: 10.19080/IJESNR.2018.13.555852

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