Impact of in Vitro Heat Shock (42.50c) on Prostaglandins, Ionic and Metabolic Contents in Sheep Endometrial Epithelial Cells

Sukanta Mondal, Avantika Mor, Sumanta Nandi and Ippala Janardhan Reddy
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Ippala Janardhan Reddy: ICAR-National Institute of Animal Nutrition and Physiology, India

Current Trends in Biomedical Engineering & Biosciences, 2017, vol. 3, issue 1, 17-19

Abstract: Elevated temperature is one of the major factors responsible for reduced fertility in domestic ruminants including sheep. The aim of the study was to delineate the effect of heat shock on prostaglandins, ionic and metabolic contents of sheep endometrial epithelial cell in vitro. Endometrial epithelial cells were cultured in RPMI 1640 medium at 38.5 °C in CO2 incubator for 24hr (5% CO2 in air, 90-95% relative humidity; Control culture; n=6). Heat stressed cultures (n=6) were acclimated at 38.50C for 6 hr and then placed at 42.5 °C for 18 hr. In vitro heat stress (42.5 °C) significantly (P 0.05) protein, phosphorus, urea, SOD, PGF2a levels in epithelial cells as compared to those exposed to 38.5 °C. It is concluded that heat shock altered prostaglandins, ionic and metabolic contents of endometrial epithelial cells in vitro.

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Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jctbeb:v:3:y:2017:i:1:p:17-19

DOI: 10.19080/CTBEB.2017.03.555604

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