Modulation of Radioresponse by an Isoquinoline Alkaloid Berberine in Cultured HeLa Cells Exposed to Different Doses of γ –Radiation

Ganesh Chandra Jagetia and Rao Sk
Additional contact information
Ganesh Chandra Jagetia: Department of Zoology, Mizoram University, India
Rao Sk: Department of Pharmacognosy, C. U. Shah College of Pharmacy & Research, India

Current Trends in Biomedical Engineering & Biosciences, 2017, vol. 8, issue 1, 1-8

Abstract: The radiomodulatory effect of berberine chloride (BCL) was studied in cultured HeLa cells exposed to γ-rays, where optimum treatment duration of BCL for its radiosensitizing activity was determined by treating HeLa cells with BCL for different times before exposure to 2Gy γ-rays. Exposure of HeLa cells to BCL for 4h before exposure to 2Gy, caused a significant decrease in the cell viability (50%) and surviving fraction (0.42) after 4h treatment with 4μg/ml BCL. The radiosensitizing activity was studied by treating HeLa cells for 4h with diffrent cocentrations of BCL before expsoure to 0, 0.25, 0.5, 1, 2, 3, or 4Gy γ-rays. Exposure of HeLa cells to different doses of γ-rays resulted in a dose dependent decline in the viability of HeLa cells, while treatment of HeLa cells with various concentrations of BCL further decreased the cell viability and clonogenic potenial of cells depending not only on the irradiation dose but also BCL concentration dependent manner. The lowest cell viability and clonogenicity was observed for 4μg/ml BCL for all exposure doses.

Keywords: juniper publishers group; juniper publishers Biomedical Engineering journals; Biomedical Engineering journals list; medical devices journals; peer reviewed Biomedical Engineering journals; open access Biomedical Engineering journals; Biomedical Engineering journals impact factor; Biomedical Engineering scientific journals; Biomedical Engineering articles; scholarly Biomedical Engineering journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://juniperpublishers.com/ctbeb/pdf/CTBEB.MS.ID.555726.pdf (application/pdf)
https://juniperpublishers.com/ctbeb/CTBEB.MS.ID.555726.php (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:adp:jctbeb:v:8:y:2017:i:1:p:1-8

DOI: 10.19080/CTBEB.2017.08.555726

Access Statistics for this article

Current Trends in Biomedical Engineering & Biosciences is currently edited by Sophia Mathis

More articles in Current Trends in Biomedical Engineering & Biosciences from Juniper Publishers Inc.
Bibliographic data for series maintained by Robert Thomas ().