Optimization of Sperm Culture Conditions for Human Assisted Reproductive Technologies

Carol L Curchoe and Charles L Bormann
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Carol L Curchoe: San Diego Fertility Center, San Diego, USA
Charles L Bormann: Harvard Medical School, Obstetrics/Gynecology/Reproductive Endocrinology and Infertility, USA

Global Journal of Reproductive Medicine, 2018, vol. 3, issue 5, 98-102

Abstract: Optimizing sperm performance can be a differentiating factor in clinical pregnancy rates for assisted reproductive technology (ART) laboratories. Minimizing cellular stress during routine sperm processing by maintaining a precise and stable environment is of great importance in the field. In commercial media, pH of the external cellular culture environment (pHe) is typically maintained at 7.2-7.4 by various buffering reagents, while the internal pH of the cell (pHi) depends on available lactate and amino acids. Combining buffers in solution has been previously shown to be valuable for stabilizing internal and external pH for various biological systems. We hypothesized that a dual-buffer culture medium might improve sperm performance for ART. Here we demonstrate superior performance of a commercially available dual-buffer solution of HEPES and MOPS: Multipurpose Handling Medium (MHM, Irvine Scientific). Significantly better performance, assessed at 8 and 48 hours, and measured as: sperm viability, total motility, and rapid forward progression, was observed for MHM over single buffer controls.

Keywords: Journal of Reproductive Medicine; Reproductive Medicine; journal of reproductive medicine; reproductive medicine articles; reproductive medicine articles impact factor; reproductive medicine research journals; reproductive journals list; open access; juniper publishers journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jgjorm:v:3:y:2018:i:5:p:98-102

DOI: 10.19080/GJORM.2018.03.555625

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