GC-MS and HPLC Analysis of Chlorophytum Borivilianum (Safed Musli) A Plant from Ayurveda- Herbal Viagra

Ruchi Vyas, Garima Sharma, Adya Chaturvedi, Devki and Rashmi Sisodia
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Rashmi Sisodia: Department of Zoology, University of Rajasthan, Jaipur, India

Global Journal of Reproductive Medicine, 2020, vol. 7, issue 5, 101-106 Volu2m: 7

Abstract: The objective of this study was to determine the photochemical content of Chlorophytum borivilianum by GC-MS analysis and HPLC. Chlorophytum borivilianum is an endangered medicinal plant having several phyto constituents, which makes it suitable for many therapeutic applications. GC-MS analysis was carried out on a Shimadzu GCMSQP2010 Ultra system. Methanol is used as a solvent. The injector temperature was 280°C. The samples were injected in the split mode with split ratio 1 to 60. Injection volume was 1 μl. A capillary column Rtx-5MS (5% Diphenyl-95% Dimethyl Polysiloxane), 30 m x 0.25mm x 0.25 μm, was used. The carrier gas was helium with a constant flow of 1.00 ml/min.GC-MS analysis of drug led to the identification of 9 compounds viz., 5-methylhex-2-yl pentadecyl ester (23.69%), 9,10-Anthracenedione (19.02%), Phthalic acid (18.63%) etc. High-performance liquid chromatography was conducted to enumerate active ingredient in the plant extracts. These analyses help us to understand the importance of various phyto chemicals and also open up new ways to exploit a particular plant for various therapeutic and restorative purposes.

Keywords: Journal of Reproductive Medicine; Reproductive Medicine; journal of reproductive medicine; reproductive medicine articles; reproductive medicine articles impact factor; reproductive medicine research journals; reproductive journals list; open access; juniper publishers journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jgjorm:v:7:y:2020:i:5:p:101-106

DOI: 10.19080/GJORM.2020.07.555723

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