Development of Analytical Method for Simultaneous Estimation of Adapalene and Benzoyl Peroxide in Gel Formulation by RP-HPLC

Harikishor Barange, Suhail Asghar and Ujami Sheikh
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Ujami Sheikh: Unijules Life Sciences Ltd, India

Global Journal of Pharmacy & Pharmaceutical Sciences, 2018, vol. 4, issue 3, 63-69

Abstract: A simple, accurate, rapid and precise isocratic high-performance liquid chromatographic method has been developed and validated for simultaneous estimation of Adapalene and Benzoyl Peroxide in pharmaceutical gel formulation. The chromatographic separation was carried out at Analytical Technology HPLC instrument (Software: HPLC Work Station) equipped with Deuterium lamp as detector, HPLC pump and manual injecting facility programmed at 20μL capacity per injection was used. The stationary phase was Zodiac Column C8 (250mmx4.6mm, 5μm) at ambient temperature. Mobile phase containing Acetonitrile: Methanol (90:10). The mobile phase flow rate 1.0mL/min, Injection volume 20μL and detection was carried out at 245nm using UV-Detector. The retention time was found to be 3.7±0.1mins and 5.8±0.1mins Benzoyl Peroxide and Adapalene respectively.

Keywords: juniper publishers:Journal of Pharmacy; Global Journal of Pharmacy; Pharmaceutical Sciences; Pharmacy & Pharmaceutical Sciences; pharmaceutical sciences journals; omics online; open access; drug discovery; Clinical Trials; juniper publishers open access journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jgjpps:v:4:y:2018:i:3:p:63-69

DOI: 10.19080/GJPPS.2018.04.555639

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