Neurophysiological Study of Traumatic Brain Injury To Slice or Not to Slice

Jianli Sun and Kimberle M Jacobs
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Jianli Sun: Department of Anatomy & Neurobiology, Virginia Commonwealth University, USA
Kimberle M Jacobs: Department of Anatomy & Neurobiology, Virginia Commonwealth University, USA

Open Access Journal of Neurology & Neurosurgery, 2017, vol. 2, issue 5, 101-106

Abstract: Diffuse axotomy is characteristic of mild traumatic brain injury (TBI) both clinically and in experimental models. Axotomy also occurs during preparation of acute brain slices for physiological recordings. Brain slices have been used for decades and have many advantages, including the ability to select cells within particular locations or with specific morphologies for study. The question addressed here is whether creating additional axotomy by preparing brain slices introduces additional injury that is already typical of TBI. We have previously demonstrated alterations in both intrinsic membrane and synaptic properties recorded in brain slices after mTBI. Here we examined whether axotomized neurons differ from intact neurons in slices made from naïve mouse pups. There was no significant difference in either excitatory synaptic postsynaptic currents or in any measures of intrinsic membrane and cellular properties. These results suggest that creating the brain slices does not induce alterations similar to that observed within two days of mTBI induced by fluid percussion injury. Thus ex vivo slices are an appropriate methodology to study the effects of TBI.

Keywords: Open Access Journal of Neurology; Journal of Neurology & Neurosurgery; Open Access Journal; juniper publishers; neurology open access journals; journal of neurology; neurology indexed journals; neurology scholarly journals; journal of neurology; neurology journals impact factor; juniper publishers reivew (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:joajnn:v:2:y:2017:i:5:p:101-106

DOI: 10.19080/OAJNN.2017.02.555600

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