The Synergism of a Novel Thermostable Acetyl Xylan Esterase with Cellulase Degrade Wheat Straw

Xiaoshen Zhao, Jin Xu, Liyang Liu, Zujun Deng, Shan Liu, Jeonyun Yun, Xiong Xiao and He Li
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Jin Xu: School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, China
Liyang Liu: Guangdong Key Laboratory of Bioactive Drug Research, China
Jeonyun Yun: Business School of Medicine, Guangdong Pharmaceutical University, China
He Li: Guangzhou Yachun Cosmetics Manufacturing Co., Ltd, China

Organic & Medicinal Chemistry International Journal, 2020, vol. 10, issue 2, 73-81

Abstract: Acetyl xylan esterase is one of the key enzymes in xylan degradation enzyme system. However, acetyl xylan esterase from natural microorganisms has low expression, low enzyme activity and impure product. In this study, a new xylanase gene, est1051, from the metagenomic library, was expressed in prokaryotic system. Enzymatic properties, including optimum temperature and pH, thermal and pH stability, organic solvent, metal ion and salt solution tolerance were explored. Finally, the synergism with cellulase on degrading straw was identified and evaluated. EST1051 displayed high homology with acetyl xylan esterases in amino acid sequences and conserved active sites. And EST1051 showed good stability across a broad temperature range, its enzymatic activities had been retained more than 50% between 4℃ and 50℃ after 24 hours of incubation.

Keywords: juniper publishers:Medicinal Chemistry; Biochemistry Journal; Pharmacology Journal; biochemical pharmacology; molecular pharmacology; biochemistry; open access journals; peer reviewed journals; Open Access; Journal of Molecular Biochemistry; International Research Journal of Pure and Applied Chemistry; World Journal of Biological Chemistry; Biochemistry and Analytical Biochemistry; Biochemistry & Physiology: Open Access; Molecular Biology International; Plant Biochemistry & Physiology; International Journal of Molecular Sciences; Journal of Clinical and Medical Genomics; Environmental Analytical Chemistry; Medicinal Chemistry; Juniper publishers reivews (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jomcij:v:10:y:2020:i:2:p:73-81

DOI: 10.19080/OMCIJ.2020.10.555785

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