Synthesis and Evaluation of Chitosan Derivatives for Large Size DNA Delivery

Sankov V, Shagdarova B, Chudakov D, Reshetov P, Grechikhina M, Zubareva A, Varlamov V, Esipov R, Zubov V and Svirshchevskaya E
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Svirshchevskaya E: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia

Organic & Medicinal Chemistry International Journal, 2017, vol. 1, issue 4, 172-178

Abstract: Chitosan (Chi) is a natural inexpensive renewable biodegradable cationic polymer with promising potency for different applications including gene delivery. Multiple studies demonstrate that unmodified Chi shows poor cellular uptake leading to low transfection efficiency. Chi possesses multiple reactive groups used to obtain derivatives with modified properties. Numerous attempts in Chi modification demonstrated improved transfection efficacy. However a direct comparison of different Chi derivative was not done. This paper analyses the efficacy of large size DNA delivery by 24 Chi. samples. A рRFP pmKate2 reporter plasmid with MW 3065 kDa was used as a model DNA to transfect HEK293 cells. Among all only a set of hexanoyl-chitosan with molecular weight 20 kDa and substitution degree 10/15/30% was able to deliver the plasmid into cells at a significant level comparable with the efficacy of liposomal reagent Metafectene Pro.

Keywords: juniper publishers:Medicinal Chemistry; Biochemistry Journal; Pharmacology Journal; biochemical pharmacology; molecular pharmacology; biochemistry; open access journals; peer reviewed journals; Open Access; Journal of Molecular Biochemistry; International Research Journal of Pure and Applied Chemistry; World Journal of Biological Chemistry; Biochemistry and Analytical Biochemistry; Biochemistry & Physiology: Open Access; Molecular Biology International; Plant Biochemistry & Physiology; International Journal of Molecular Sciences; Journal of Clinical and Medical Genomics; Environmental Analytical Chemistry; Medicinal Chemistry; Juniper publishers reivews (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jomcij:v:1:y:2017:i:4:p:172-178

DOI: 10.19080/OMCIJ.2017.01.555572

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