Method Development and Validation for the Estimation of Aliskiren in Pharmaceutical Dosage Form by RP-HPLC

Yadav Nita and Goyal Anju
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Yadav Nita: Department of Pharmacy, SRMSCET, India
Goyal Anju: B N Institute of Pharmaceutical Sciences, India

Organic & Medicinal Chemistry International Journal, 2018, vol. 5, issue 3, 86-90

Abstract: A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Aliskiren in tablet dosage form. A C8 Chromasil column (150 × 4.6 mm, 5μm particle size) was used as a stationary phase with a mobile phase containing a mixture of phosphate buffer, methanol and ACN in ratio of 55:10:35, v/v. The flow rate was 1.0 mL/min. The effluent was monitored at 220 nm and eluted at 2.342 min. Calibration curve was plotted with a range from 30-150 μg/ml for Aliskiren and the correlation was found to be 0.999. The accuracy range was found between 100.06-100.43%. The % RSD values for both intraday and interday precision were less than 2.0. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 2.335μg/ml and 7.077μg/ml respectively. The assay was validated for the parameters like system suitability, precision, accuracy, and robustness parameters. The proposed method can be useful for the routine determination of Aliskiren in pharmaceutical dosage form.

Keywords: juniper publishers:Medicinal Chemistry; Biochemistry Journal; Pharmacology Journal; biochemical pharmacology; molecular pharmacology; biochemistry; open access journals; peer reviewed journals; Open Access; Journal of Molecular Biochemistry; International Research Journal of Pure and Applied Chemistry; World Journal of Biological Chemistry; Biochemistry and Analytical Biochemistry; Biochemistry & Physiology: Open Access; Molecular Biology International; Plant Biochemistry & Physiology; International Journal of Molecular Sciences; Journal of Clinical and Medical Genomics; Environmental Analytical Chemistry; Medicinal Chemistry; Juniper publishers reivews (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jomcij:v:5:y:2018:i:3:p:86-90

DOI: 10.19080/OMCIJ.2018.05.555663

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