Vitamin C is Required To Allow Sustained IL-1β-Induced Suppression of The Pyrophosphate Regulators ANKH and ENPP1 in Bovine Nucleus Pulposus Cells

Robert J Frawley, AgataKrzyzanowska Ms and Matthew E. Cunningham
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Matthew E. Cunningham: Hospital for Special Surgery, USA

Orthopedics and Rheumatology Open Access Journals, 2017, vol. 5, issue 4, 95-100

Abstract: The extracellular concentration of pyrophosphate (PPi) is increased by the activity of proteins like ectonucleotidepyrophosphatase/phsophodiesterase (ENPP1) and progressive alkalosis protein homolog (ANKH), whereas the concentration of PPi is decreased by tissue non-specific alkaline phosphatase (TNAP) through hydrolysis. Since PPi prevents calcium-phosphate deposition, ANKH and ENPP1 inhibit tissue mineralization and TNAP enhances mineralization. The presence of the inflammatory cytokine Interleukin-1 (IL-1)β alters expression of important regulatory proteins in many cells. The objective was to determine the effect of IL-1β on the gene expression of ANKH and ENPP1 in bovine Nucleus pulposus (bNP) cells, and the importance of vitamin C.A dose-dependent increase in calcium staining of TNAP-transduced bNP cell cultures was observed with alizarin red, indicating increased calcium deposition in the matrix at 3 and 7 days when treated with IL-1β.

Keywords: journal of orthopaedics; orthopaedics journals impact factor; orthopaedics articles impact factor; scholarly publishing orthopedics journals; juniper publishers opena ccess orthopedics journal; Rheumatology; rheumatology journals impact factor 2018; rheumatology journals impact factor; rheumatology open access journals; juniper publishers review (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:joroaj:v:5:y:2017:i:4:p:95-100

DOI: 10.19080/OROAJ.2017.05.555674

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