In-Vitro Conservation of Sugarcane Germplasm

Rabia Ikram, Shazia Erum, Hina Javed, Shah Zaman, Shahid Khan, Aish Muhammad and Saima Kanwal

Asian Journal of Agriculture and Rural Development, 2014, vol. 04, issue 11, 4

Abstract: Lack of in vitro multiplication procedure has long been a serious problem in sugarcane breeding programs. This problem has been solved to maximum extent by In Vitro propagation. In the present study, 6 genotypes of sugarcane (SC 06, SC 04, SC 25, SC 11, SC 13 and SC 30) was acquired & excised for apical and sub apical parts (eyes/ lateral buds). The explants were surface sterilized with Clorox (70% +100%, 20 & 10 min). Results showed that highest survival % was achieved with apical buds (66%) as compared to lateral buds (33%). In addition, effect of different concentrations of rooting media (NAA, 5% Sucrose and 6% Sucrose) and shooting media (GA3, 0.1mg/, Kinetin, 0.1mg/L and BAP 0.1mg/L, Kinetin 0.1mg/L,GA3 0.1mg/L, NAA 0.1mg/L) was studied. Synergistic response was observed on the growth of plant with hormone consortia. Significant differences were observed in No. of roots, shoot length @ No. of shoots at all tested treatments. Highest No. of roots recorded for SC 30 at with 6% Sucrose. Highest No. of shoots was recorded for SC 04 with GA3 @ 0.1mg/L and highest shoot length for SC 06 at 0.1mg/ L of Kinetin.

Keywords: Agricultural and Food Policy; Industrial Organization (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:ags:ajosrd:198431

DOI: 10.22004/ag.econ.198431

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