IN VITRO HOST-FREE CALLUS DEVELOPMENT AND PLANT REGENERATION OF OROBANCHE CRENATA FORSK. AN OBLIGATE ROOT-PARASITE

Mounia Ennami, Houda Taimourya, Fatima zahra Briache, Rabha Abdelwahd, Lamiae Ghaouti, Loubna Belqadi and Rachid Mentag

International Journal of Agriculture and Environmental Research, 2024, vol. 10, issue 03

Abstract: This study investigates the development of an in vitro system for callus induction, root-like protrusion formation, and plant regeneration in Orobanche crenata, a parasitic plant of economic significance to legume crops. The research aims to establish a controlled environment for studying O. crenata biology and physiology independent of host plants, offering insights into its growth dynamics and potential applications in parasitic weed management. Various culture media and growth regulators were tested to optimize conditions conducive to callus initiation and subsequent differentiation. Results demonstrate that B5 medium supplemented with gibberellic acid (GA) at 20 mg L-1 effectively induces callus formation and supports the development of root-like structures, crucial for further studies on host-parasite interactions and potential therapeutic applications.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:ags:ijaeri:344632

DOI: 10.22004/ag.econ.344632

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