The Use of a Rabbit Model to Evaluate the Influence of Age on Excision Wound Healing

S. Bilal, S.A. Bhat, I. Hussain, J.D. Parrah, S.P. Ahmad and M.R. Mir

Journal of Life Sciences Research, 2015, vol. 2, issue 3, 72-75

Abstract: Background: The wound healing involves a highly co-ordinated cascade of cellular and immunological response over a period of time including coagulation, inflammation, granulation tissue formation, epithelialization, collagen synthesis and tissue remodeling. Wounds in aged heal more slowly than those in younger, mainly because of co morbidities that occurs as one ages. Present study is about the influence of age on wound healing. 1x1cm2 (100mm) wounds were created on the back of the animal. The animals were divided into two groups; one group had animals in the age group of 3-9 months while another group had animals in the age group of 15-21 months. Materials and Methods: 24 clinically healthy rabbits in the age group of 3-21 months were used as experimental animals and divided in two groups viz A and B. All experimental parameters i.e Excision wound model, Measurement of wound area, Protein extraction and estimation, Protein extraction and estimation and DNA extraction and estimation were done by standard methods. Results: The parameters studied were wound contraction, hydroxyproline, glucosamine, protein and DNA. Significant increase (p<0.005) in the hydroxyproline, glucosamine, protein and DNA and significant decrease in wound area (p<0.005) was observed in the age group of 3-9 months when compared to animals of age group of 15-21 months. Wound contraction together with hydroxyproline, glucosamine, protein and DNA estimations suggest that advance age results in retarded wound healing. Conclusion: The decrease wound contraction and accumulation of hydroxyproline, glucosamine, protein and DNA in group B animals may be associated with the reduction or delay in growth factors because of the advancing age.

Keywords: Age; Wound healing; Excision wound; Hydroxyproline; Glucosamine. (search for similar items in EconPapers)
Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
http://asianonlinejournals.com/index.php/Lifsc/article/view/626/622 (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:aoj:lifscr:v:2:y:2015:i:3:p:72-75:id:626

Access Statistics for this article

More articles in Journal of Life Sciences Research from Asian Online Journal Publishing Group
Bibliographic data for series maintained by Sara Lim ().