High-throughput Sequencing Analysis and Function Prediction of Lung Microbiota in Healthy Rats

Xiaoyu Li*, Zhibang Yang, Jia Liu and Ying Jiang
Additional contact information
Xiaoyu Li*: Laboratory of Innovation, Basic Medical Experimental Teaching Center, Chongqing Medical University, Chongqing, China
Zhibang Yang: Laboratory of Pathogenic Biology and Immunology, School of Basic Medicine, Chongqing Medical University, Chongqing, China
Jia Liu: Basic Medical Experimental Teaching Center, Chongqing Medical University, Chongqing, China
Ying Jiang: Laboratory of Pathogenic Biology and Immunology, School of Basic Medicine, Chongqing Medical University, Chongqing, China

International Journal of Healthcare and Medical Sciences, 2019, vol. 5, issue 3, 12-17

Abstract: To form the basis of a respiratory disease model in rats by investigating the microbial distribution and composition in the lower respiratory tracts of normal rats. Methods: DNA was extracted from the intestine, trachea, bronchus and lung samples collected from healthy rats under sterile conditions. The 16S rDNA V4-V5 region was sequenced using Illumina high-throughput technology. Results: The sequencing results showed that there was no significant difference in abundance and species diversity of microbiota between the lower respiratory and the intestine. The microbiota structure analysis showed samples from lungs and intestinal shared similarity. However, the dominant species at the levels of phylum, family, and genus diverged. The similarity analysis showed that the lung microbiota were different from the intestines. The linear discriminant analysis showed significantly different species in different tissues; function prediction also showed different microbiota function in different tissues. Conclusions: These results suggest that bacterial colonization depends on the sample’s anatomical location. The human pathogen Acinetobacter lwoffii was also detected in the rat lower respiratory tract samples.

Keywords: Illumina sequencing; Lung microbiota; Lower respiratory tract; Intestinal; 16S rRNA; Rats. (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.arpgweb.com/pdf-files/ijhms5(3)12-17.pdf (application/pdf)
https://www.arpgweb.com/journal/13/archive/03-2019/3/5 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:arp:ijohms:2019:p:12-17

DOI: 10.32861/ijhms.53.12.17

Access Statistics for this article

International Journal of Healthcare and Medical Sciences is currently edited by Dr. Bonyah, Ebenezer

More articles in International Journal of Healthcare and Medical Sciences from Academic Research Publishing Group Rahim Yar Khan 64200, Punjab, Pakistan.
Bibliographic data for series maintained by Managing Editor ().