Genetic Diversity of White Yam ( Dioscorea rotundata Poir) Accessions Maintained in Tissue Culture and Cultivated in Field using Simple Sequence Repeat (SSR) Markers and Morphological Characterization

Abu Gabriel, Paterne Agre, Alex Edemodu, A. E. Okon, Ranjana Bhattacharjee and David De Koyer
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Abu Gabriel: Department of Genetics and Biotechnology University of Calabar, Nigeria
Paterne Agre: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria
Alex Edemodu: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria
A. E. Okon: Department of Plant Science and Biotechnology, Faculty of Biological Sciences, CRUTECH, Calabar, Nigeria
Ranjana Bhattacharjee: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria
David De Koyer: Agriculture and Agri-Food Canada, Fredericton, New Brunswick, Canada

Journal of Agriculture and Crops, 2021, vol. 7, issue 2, 60-68

Abstract: Dioscorea rotundata is a staple food crop for millions of people in the tropical and subtropical regions. In vitro germplasm conservation is a very useful tool in yam improvement strategies but very little is known about the genetic integrity and stability of in-vitro conserved yam plants. In this study, 42 accessions from in vitro and field populations were genotyped using 11 microsatellite markers and 23 morphological descriptors to assess variability within and between accessions. Out of the 23 morphological variables used, 13 were identified as most discriminate and were used to cluster the accessions into 4 clusters using the unweighted pair group arithmetic mean average (UPGMA). Accession maintained in field as well as in in-vitro showed high genetic similarity (R2 = 0.91, p-value: 1e-04). Out of the 42 accessions analyzed, nine accessions maintained in the field and in-vitro displayed different genetic profiles. This study provided basic information on the possible somaclonal variation of yam accessions maintained through in-vitro. Further study with advanced tools such as next-generation sequencing is required to elucidate the nature of the observed variation within clones.

Keywords: Dioscorea rotundata; Field; Genetic diversity; Morphological characterization; In vitro; Simple sequence repeats (SSR) (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:arp:jacarp:2021:p:60-68

DOI: 10.32861/jac.72.60.68

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