Evaluation of Extraction Methods of DNA from Dry Collection Material of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae)

Fahriye Sumer Ercan, Neslihan Bayrak () and Sevim Dogan
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Fahriye Sumer Ercan: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Ahi Evran University, 40200, Kırşehir, Turkey
Neslihan Bayrak: Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Bozok University, 66900, Yozgat, Turkey
Sevim Dogan: Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Bozok University, 66900, Yozgat, Turkey

Journal of Biotechnology Research, 2016, vol. 2, issue 1, 1-5

Abstract: Different DNA extraction protocols are evaluated for DNA isolation from various samples. In our study we compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocols obtained from dry collected materials of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae) samples. Although, the hihgest yield of DNA was obtained from C100 method, the purest DNA was obtained with Qiagen protocol. Using RAPD-PCR, we demonstrate the efficacy of Qiagen protocol on these samples collected up to 6 years ago.Â

Keywords: Urophora cuspidata; Urophora macrura; Tephritidae; DNA extraction; Chelex; RAPD-PCR (search for similar items in EconPapers)
Date: 2016
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