Construction, Expression, and Purification of ZNF191 (243-368) Zinc Finger Deletion Mutants

Dongxin Zhao* and Zhongxian Huang
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Dongxin Zhao*: College of Chemistry, Chemical and Environmental Engineering, Henan University of Technology, Zhengzhou, Henan, China
Zhongxian Huang: Department of Chemistry, Fudan University, Shanghai, China

Journal of Biotechnology Research, 2018, vol. 4, issue 11, 80-82

Abstract: The C-terminal region of the zinc finger protein ZNF191(243-368) contains a putative DNA-binding domain containing four Cys2His2 zinc finger motifs. To understand the properties and functions of the zinc finger motifs, a deletion gene of ZNF191(243-368) was inserted into pGEX-4T-2. The recombinant vector was transformed into Escherichia coli BL21, and a glutathione S-transferase (GST) fusion protein was expressed and purified using glutathione agarose affinity resin. The results show that this expression system can be used to express and purify zinc finger deletion mutants of ZNF191(243-368), providing a basis for further investigation of this protein.

Keywords: Affinity purification; Deletion mutant; GST fusion protein; Zinc finger protein; Recombinant vector. (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:arp:rjbarp:2018:p:80-82

DOI: 10.32861/jbr.411.80.82

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