Calcite-Forming Bacteria Located in Limestone Area of Malaysia

T Komala and Tan C Khun

Journal of Asian Scientific Research, 2013, vol. 3, issue 5, 471-484

Abstract: Calcite-forming by bacteria has been reported in various geological environments including limestone caves, and soil. There are four natural processes by which calcite is formed: carbonic anhydrase (CA), sulphate reduction, nitrate reduction, and urea hydrolysis. The aim of study is to identify calcite-forming bacteria occurring in limestone areas of Malaysia. Ten bacilli were identified to capable of calcite forming out of 23 total isolates. The processes of calcite forming were found to be either CA or urea hydrolysis, as none of the isolates falls into nitrate or sulphate reducing type of calcite-forming bacteria. Identification using 16S ribosomal DNA gene sequencing with polymerase chain reaction (PCR) identified B.sphaericus, B.cereus and B. pumilus; B.sphaericus, and B.cereus falls into urea hydrolysis and CA type, whereas B.pumilus falls into CA type of calcite forming.

Keywords: Calcite-forming bacteria; Limestone areas; Carbonic anhydrase; Urea hydrolysis (search for similar items in EconPapers)
Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://archive.aessweb.com/index.php/5003/article/view/3505/5580 (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:asi:joasrj:v:3:y:2013:i:5:p:471-484:id:3505

Access Statistics for this article

More articles in Journal of Asian Scientific Research from Asian Economic and Social Society
Bibliographic data for series maintained by Robert Allen ().