Comparison Survey of Receiving the Plasmid 253pil by Bacillus Cereusatcc1098 and Bacillus Cereus ATCC14579 by Using of Electroporation and Thermal Shock Method

Ciamak Ghazaei

Journal of Asian Scientific Research, 2016, vol. 6, issue 12, 169-173

Abstract: According to the studies conducted, Bacillus cereus is a gram-positive aerobic bacterium which is able to produce spores. B. cereus is involved in formation of microflora. Currently, some strains of B. cereus are used as probiotics in animal and poultry feed. Genetic investigation is considered as an important objective of biotechnological and microbiological studies. This study compares the received plasmid 253pIL by B. cereus ATCC14579 and B. cereus ATCC1098. There are several methods for transformation; this study used chemical method (thermal shock) and electroporation. Due to low efficiency of these two methods in B. cereus strains, the protoplast of two B. cereus strains ATCC14579 and ATCC10987 were used in this study. Finally, these two strains were compared for two different transformation methods. The results showed that both strains ATCC1098 and ATCC14579 received more plasmid via electroporation.

Keywords: Bacillus cereus; Electroporation; Thermal shock; Transformation; Heat shock; Plasmid. (search for similar items in EconPapers)
Date: 2016
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