Proteomic and Genomic Techniques in Medical Research: Applications in Cancer, Diagnostics, And Personalized Medicine

Oghenetega E. Imiruaye and Liasu Ogunkanmi
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Oghenetega E. Imiruaye: Keck Graduate Institute, United States of America (USA)
Liasu Ogunkanmi: Keck Graduate Institute, United States of America (USA)

International Journal of Latest Technology in Engineering, Management & Applied Science, 2025, vol. 14, issue 6, 959-967

Abstract: Advancements in proteomic and genomic technologies have transformed molecular biology by enabling comprehensive analysis of biological systems at the molecular level. This literature review explores the evolution, methodologies, and practical applications of key proteomic and genomic techniques. In proteomics, tools such as two-dimensional electrophoresis, mass spectrometry, Western blotting, Edman degradation, and functional protein microarrays have facilitated high-throughput protein identification, post-translational modification analysis, and biomarker discovery. Similarly, genomic methodologies like PCR, recombinant DNA technology, gel electrophoresis, and Southern blotting have revolutionized gene detection, manipulation, and expression profiling. The review also highlights the interdisciplinary impact of these technologies across clinical diagnostics, oncology, autoimmune disorders, infectious disease surveillance, cardiovascular research, and personalized nutrition. Integrative approaches combining proteomics and genomics are enabling the discovery of novel therapeutic targets, improving disease classification, and advancing precision medicine. Despite current limitations, such as the absence of amplification techniques for proteins and challenges in data interpretation, ongoing innovations promise to bridge these gaps. This synthesis underscores the pivotal role of molecular techniques in deepening our understanding of human biology and accelerating biomedical advancements for improved healthcare outcomes.

Date: 2025
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