A Pilot Study of the Antibacterial Activity of Garlic (Allium Sativum) Against Selected Drug Resistant Organisms

Igwe Onyekachi Fidelis, Tamunotonye Abel Briggs, Dike Uzoma Awa, Okata-Nwachukwu Maria Okwudili, Anozie Justina Kenechukwu, Akpangen Chineme Mayfair and Ugboaja Felicia Chijimdu
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Igwe Onyekachi Fidelis: Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State
Tamunotonye Abel Briggs: Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State
Dike Uzoma Awa: Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State
Okata-Nwachukwu Maria Okwudili: Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State
Anozie Justina Kenechukwu: Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State Department of Food Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B.1007. Afikpo. Ebonyi State
Akpangen Chineme Mayfair: Microbiology Option, Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State
Ugboaja Felicia Chijimdu: Microbiology Option, Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic Unwana, P.M.B. 1007. Afikpo. Ebonyi State

International Journal of Research and Scientific Innovation, 2025, vol. 12, issue 15, 618-637

Abstract: The objective of this research was to determine the antibacterial effects of the aqueous and ethanol extracts of Allium sativum (Garlic) against selected drug resistant organisms of Staphylococcus spp and Escherichia coli. We carried out antibacterial tests on the selected drug resistant organisms using paper disc diffusion by kirby-bauer disc diffusion method. Whiteman filter paper was cut into pieces of 6mm and dipped into different concentrations of the extracts that were serially diluted, allowed for proper diffusion for 30minutes and placed on the pre-inoculated plates of the test organisms, incubated for 24hours after which zone of inhibition diameter (ZID) were measured in MM. The results indicated that 400mg/ml concentration and the control antibiotics(AUG-30mg) were effective against staphylococcus spp and Escherichia coli for both aqueous and ethanol extracts with zone of inhibition of 15,15,10,14,20,23,10,13 respectively. the 200mg/ml of both aqueous and ethanol extracts was also effective against the test organisms with zones of inhibition diameter of 0,13,13,0 for the resistant strains of Staphylococcus aureus and Escherichia coli respectively.while the concentrations of 100mg/ml and 50mg/ml didn’t show any zone of inhibition against the test organisms.the MIC and MBC for Staphylococcus aureus in both extracts is 400mg/ml while MIC for Escherichia coli in both extracts is 200mg/ml and MBC IS 400mg/ml. Both the control and different concentrations of 400mg/ml and 200mg/ml of the extracts were active against the selected resistant organisms while some strains were resistant to the extracts especially to the cocentrations of 100mg/ml and 50mg/ml. The result showed that Garlic could be very effective as an antibacterial agent against drug resistant organisms of Staphylococcus aureus and Escherichia coli mostly at a high concentration dosages and dependent on the type of drug resistant organism.

Date: 2025
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