Quantification of Glycyrrhizin from Different Extract of Glycyrrhiza Glabra

Clariya Rapheal and Prasanth S.s
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Clariya Rapheal: Department of Pharmaceutical chemistry, Westfort College of pharmacy
Prasanth S.s: Department of pharmaceutical analysis, Alshifa college of pharmacy

International Journal of Research and Scientific Innovation, 2021, vol. 8, issue 3, 112-115

Abstract: Identification and quantification of phytoconstituents in Glycyrrhiza glabra was carried out by HPTLC. Whereas the dried root of Glycyrrhiza glabra was extracted successively with solvent like hexane, chloroform and methanol respectively. The phytochemical screen of each extract was performed and compares the result. The Total phenolic content of each extract were carried out using Gallic acid as reference. In that the methanolic extract had a higher amount of total phenolic content.. The anti oxidant activity of each extract were carried out by DPPH assay using ascorbic acid as reference standard. This assay revealed that methanolic extract had a lower IC 50 value .The quantification of glycyrrhizin in each extract was carried out by HPTLC using silicagel F254nm plate which was developed in optimized solvent system of Chloroform:Methnol:water (6:3.5:0.5v/v/v).HPTLC revealed that methanolic extract of Glycyrrhiza glabra was having higher amount of Glycyrrhizin content.

Date: 2021
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