Motility, Viability and Fertility of Indonesian Native Rooster Spermatozoa in Skim-Milk Based Semen Extender

Dadang Mulyadi Saleh and Agus Susanto
Additional contact information
Dadang Mulyadi Saleh: Faculty of Animal Science, University of Jenderal Soedirman, Purwokerto, Central Java, Indonesia
Agus Susanto: Faculty of Animal Science, University of Jenderal Soedirman, Purwokerto, Central Java, Indonesia

International Journal of Research and Innovation in Applied Science, 2025, vol. 10, issue 1, 463-467

Abstract: The purpose of this study was to determine the motility and fertility of kampung rooster spermatozoa diluted in skim-milk based semen diluent. Semen that was collected from 12 kampung roosters, collected in one tube, then divided into six treatment groups, namely P0 = semen + skim milk extender; P1= semen + skimmed milk extender + 50 mM Glucose; P2= semen + skimmed milk extender + Ringer’s Lactate (4:1); P3= semen + skimmed milk extender + Ringer Lactate + 50 mM Glucose; P4= semen + skim milk extender + Physiological saline (4:1), and P5= semen + skim milk extender + Physiological saline (4:1) + Glucose 50 mM. All treated semen samples were stored at a refrigeration temperature of 3–5°C for 1 hour. Motility and viability were then observed under a microscope. This process was repeated four times. Subsequently, a single insemination was performed in the afternoon for each treatment group, with 10 Isa Brown hens aged 50 weeks assigned per group (n=60). This study used a completely randomized design (CRD). The results showed that the average of motility (%) of each treatment was: 81.25± 2.50; 83.75±4.79; 85.00±4.10; 86.25±2.50; 86.25±2.50 and 87.50±2.89. Average of viability was: 86.25±2.63; 88.75±4.11; 90.00±3.70; 90.25±6.80; 91.00±2.70 and 92.25±2.22. Average of fertility (%) was: 83.33±6.60; 85.10±8.32; 85.41±8.32; 92.85±6.58; 84.78±6.83 and 86.67±6.78. It can be concluded that the six extenders under study did not have significant effects on motility, viability, or fertility, indicating that all six skim-milk based extenders are suitable for use as extenders for fresh Kampung rooster semen.

Date: 2025
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.rsisinternational.org/journals/ijrias/ ... -issue-1/463-467.pdf (application/pdf)
https://rsisinternational.org/journals/ijrias/arti ... ased-semen-extender/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:bjf:journl:v:10:y:2025:i:1:p:463-467

Access Statistics for this article

International Journal of Research and Innovation in Applied Science is currently edited by Dr. Renu Malsaria

More articles in International Journal of Research and Innovation in Applied Science from International Journal of Research and Innovation in Applied Science (IJRIAS)
Bibliographic data for series maintained by Dr. Renu Malsaria ().