Molecular Characterization of Isolates and Antibiotic Resistance Genes Associated with Fish Pond

C.c Niyi-David, D.n Ogbonna, N.p Akani and S.I. Douglas
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C.c Niyi-David: Department of Microbiology, Rivers State University, P.M.B 5080, Port Harcourt, Nigeria
D.n Ogbonna: Department of Microbiology, Rivers State University, P.M.B 5080, Port Harcourt, Nigeria
N.p Akani: Department of Microbiology, Rivers State University, P.M.B 5080, Port Harcourt, Nigeria
S.I. Douglas: Department of Microbiology, Rivers State University, P.M.B 5080, Port Harcourt, Nigeria

International Journal of Research and Innovation in Applied Science, 2020, vol. 5, issue 8, 11-16

Abstract: Characterization of isolates from the fish pond water collected from Port Harcourt, in Rivers State, was done using 16S rRNA gene sequencing method to identify their phylogenetic relationship. The presence of the resistance gene was investigated by polymerase chain reaction followed by agarose gel electrophoresis of the amplification products. A total of 10 bacteria were identified, they include Escherichia coli, Klebsiella quasipnemoniae, Morganella morgani, Vibrio parahaemolyticus, Pseudomonas xiamenesis, Staphylococcus sp, Bacillus flexus, Lysinibacillus macroides, Myoides odoratimimus and Chryseobacterium cucumeris. The Lysinibacillus macroides and Vibrio parahaemolyticus were found to be carriers of the CTX-M gene, while Escherichia coli carried the QNRB gene. SHV gene was also found in the Vibrio parahaemolyticus. The resistance gene found in these bacteria can be transferred to other microbes to cause antibiotic pollution which is a major health challenge.

Date: 2020
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