Production of Citric Acid from Aspergilus Niger

Nikolas Keith
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Nikolas Keith: School of Biological Sciences, University of Reading, Reading, United Kingdom

International Journal of Research and Innovation in Applied Science, 2022, vol. 7, issue 12, 01-04

Abstract: Citric acid is a commercially important product used in several industrial processes. Solid state fermentation was used in this work to produce citric acid by using a locally isolated Aspergillus niger, from spoilt solidified pap (eko). About 25g of plantain peel was gelatinized and 100m1 of nutrient solution was added. Aspergillus niger spores of about 5×108 was added. The set-up was incubated for 15 days at room temperature. 1ml of the filtrate resulting from the fermentation was taken on 6th day, 8th day and 15th day into a test-tube. Pyridine and acetic anhydride were added and heated in water bath at 32°C for 30 minutes. The absorbance was taken using 420nm as wave length. The concentration of the citric acid produced by the locally isolated Aspergillus niger on 6th day, 8th day and 15th day of incubation were 4.22g11, 2.29g/1, and 14.77g/1 respectively. This result, which demonstrate the viability of citric acid production by Aspergillus niger isolated from spoilt solidified pap (eko) using plantain peel as a substrate can be of interest to possible future industrial applications.

Date: 2022
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