Micropropagation of Mokara Orchid by Temporary Immersion System Technique

Tran Van Minh
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Tran Van Minh: International University, Vietnam National University Ho Chi Minh City, Vietnam

International Journal of Research and Innovation in Applied Science, 2022, vol. 7, issue 5, 54-58

Abstract: Based young leaves of Mokara Leuen Berger Gold were used as cultured materials. Callus was initiated and increase biomass on medium of semisolid and liquid cultures: MS medium supplemented with CW (30%), sucrose (30 g/l), 2.4D (1 mg/l). Callus was used as materials for initiation and biomass propagation on medium: (1) MS medium supplemented with NAA (1 mg/l), B1 (5 mg/l), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (30 g/l) (2) MS medium supplemented with 2.4D (1 mg/l), CW (30%), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (30 g/l). Semisolid medium for callus cell regeneration and to induce multiple shoots were: MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (20 g/l). Multiple-shoots were propagated on MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Propagation of multiple-shoots in TIS on MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Interval culture time was optimum for 2 hours floating and 1 minute rising. Plantlets were induced roots on MS medium supplemented with NAA (1 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Experiments on callus formation and callus growth of mokara orchids both on semi-solid medium (agar) and liquid medium to create callus suspension, the best medium selected was: MS supplemented with 2.4D (1 mg/l), CW (30%), sucrose (30 g/l)

Date: 2022
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