Thin Cell Layer Culture of Dendrobium sp. and Cymbidium sp

Tran Van Minh
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Tran Van Minh: International University, Vietnam National University Ho Chi Minh City, Vietnam Key Lab of Plant Cell Biotechnology, Institute of Tropical Biology, Vietnam

International Journal of Research and Innovation in Applied Science, 2023, vol. 8, issue 6, 85-93

Abstract: Vietnam, a nation located in tropical region, has an important plan for flower development esspecially in tropical orchid; and the first barrier is seedling production. Some orchid species are difficult to regenerate, and thin cell layer is a method for manipulation. Dendrobium in low land tropic and Cymbidium in high land tropic were used as model to produce seedlings for high demand from farmers. Dendrobium sp.: Young shoot from the pot was used as materials for in vitro shoot regeneration on the medium MS + BA (1mg/l) + IBA (0.1mg/l) after 30 days and the in vitro shoots were cultured on the same media for multiplication. The shoots were prepared separately to leaves and shoot tip that were cultured on the thin cell layer culture medium MS + 2iP (1mg/l) + IBA (0.1mg/l) to raise 6-8 shoots/shoot tip sample and 4-6 PLB/leaf sample. Diversity of gene in cultivation was not recorded in difference about PLB initiation with 4-6 PLB/sample. Shoots and PLB were multiplied on the medium of MS + Adenine sulfate (10mg/l) + IBA (0,1mg/l) + BA (1mg/l). Shoots were rooted well on the medium MS + IBA (1mg/l). The thin cell layer culture of Dendrobium sp. was established. Cymbidium sp.: Young shoot from the pot was used as materials for in vitro shoot regeneration on the medium MS + peptone (1g/l) + BA (1mg/l) + NAA (0.5mg/l) after 30 days. In vitro shoots were used as materials and were multiplied on the medium MS + peptone (1g/l) + BA (1mg/l) + NAA (0.5mg/l). Shoots were released separately to leaf and shoot tip and cultured on the thin cell layer culture medium MS + peptone (1g/l) + BA (1mg/l) + NAA (0.5mg/l) to raise 4-6 PLB/shoot tip sample and 3-5 PLB/leaf sample. PLB raised from shoot tip was bigger than from leaf. PLBs were multiplied on the medium of MS + BA (1mg/l) + NAA (0.5mg/l). PLBs were regenerated and rooted on the medium MS + BA (0,1mg/l) + rhizogen (5mg/l) with high rate of 100% after 45 days. The diversity of gene was recognized that it was not different on 5 clones cultured about PLB initiation with 4-6 PLB/sample. The thin cell layer culture of Cymbidium sp. was established. Application of TCL method for two orchid sepcies leading to create primary materials as multiple shoots and PLB for far micropropagation.

Date: 2023
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