Synergistic Efficacy, Antimicrobial and Wound Healing Potentials of Milled Persea Americana Seed / Syzygium Aromaticum Oil Against Albino Rats and Wound Isolates

O. T. Osuntokun, Yusuf-Babatunde and O. O. Olubena
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O. T. Osuntokun: Department of Microbiology, Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria.
Yusuf-Babatunde: Department of Pharmacy Technician, Ogun State Polytechnic of Health and Allied Sciences, Ilese-Ijebu, Nigeria
O. O. Olubena: Department of Public Health Technician, Ogun State Polytechnic of Health and Allied Sciences, Ilese-Ijebu, Nigeria.

International Journal of Research and Innovation in Applied Science, 2024, vol. 9, issue 5, 554-576

Abstract: This study aimed to investigate the effects of oil avocado extracts, vegetable oil, Goya oil, and clove oil and its healing potential on male and female albino rats and their antibacterial activity against surgical wound isolates. The antimicrobial efficacy of avocado seed oil and cloves extract against surgical wound isolates was investigated using the agar well diffusion method and a microplate reader to detect the killing time rate of each isolate against oil extracts applied topically to the wounds of albino rats. The following bacteria were isolated, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Chrysobacterium meningosepticum, Klebsiella oxytoca, Escherichia coli, Acinetobacter baumannii and Bergeyella zoochelum. The wound healing process was evaluated by measuring the serum and tissue bioassays and tissue repair enzymes. It was observed that Avocado seed oil infused in vegetables has the highest micro-titre reading against Bergeyella zoochelum (0.199) and Avocado oil infused in vegetable oil with cloves (0.100) against Chrysobacterium meningo septicum at 24 and 48 hours, respectively, this denotes bacteriostatics and Avocado oil infused in Goya with cloves against Bergeyella zoochelum (0.046) has the lowest value and Avocado oil infused in n-hexane against Escherichia coli has the highest value (0.080); this denotes bacteriocide activity of extracted oil. It was observed that the surgical wound on the albino rats closed within seven days and the measured tissue and serum bioassay were relatively under standard. The results of this study suggest that avocado seed oil and clove oil have potential natural alternatives to synthetic antimicrobial agents and treatment of wounds and wound infection in albino rats, as the avocado seed oil and clove used in this study inhibited all the surgical wound isolates.

Date: 2024
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