Molecular Detection of Beta Lactamase Genes from Salmonella Typhimurium Isolated from Poultry Droppings in Nyanya, Abuja

Kanu N.V., Orji S.L., Okafor H.A., Adama R. I., F.A. Mustapha, Oluwafemi M. P., Offiong A. B., Tsebam B.C., S.c Donli and Ekwe E.o
Additional contact information
Kanu N.V.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Orji S.L.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Okafor H.A.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Adama R. I.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
F.A. Mustapha: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Oluwafemi M. P.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Offiong A. B.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Tsebam B.C.: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
S.c Donli: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria
Ekwe E.o: National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria

International Journal of Research and Innovation in Applied Science, 2024, vol. 9, issue 8, 211-222

Abstract: Salmonella infections are one of the major global public health problems. During the last decade, antibiotic resistance and multiresistance of S. typhimurium. This study aimed at molecular detection of Beta Lactamase genes from S. typhimurium Isolated from poultry droppings in Nyanya, Abuja. Fecal droppings samples were collected from two different Poultry farms using sterile screw tubes. S. Typhimurium was isolated and identified using standard microbiology methods. Antimicrobial susceptibility was carried out using Clinical and Laboratory Standards Institute method. Out of 180 samples collected the overall occurrence of S. typhimurium was 20 (11.1%). S. typhimurium isolated were higher in Farm A 9(10.0%) than in farm B 11(12.2%). The S. typhimurium isolates from Farm A were highly resistant to Ampicillin with 77.7% but less resistant to gentamicin with 33.3%, ofloxacin, ciprofloxacin and Sulphamethoxazole/trimethoprim with 44.4%. Isolates from Farm B were highly resistant to Ampicillin and Sulphamethoxazole/trimethoprim 81.8%, but less resistant to Cefuroxime 36.3%. the most common phenotypic resistant observed were C-CIP-CAZ-CRO-AUG- OFL -SXT- CEF (22.2%) from Farm A. CIP-CRO-CAZ- AMP -AUG- OFL -SXT- CEF (18.1%) from Farm B, The commonest MAR index observed in this study was 0.8(33.3%) in farm A and (36.3%) in farm B. The order of occurrences of the 4 Beta Lactamase genes detected was: blaCTX-M-9 and blaSHV (75.0%) > bla CMY2 and Bla CTX-M (50.0%). The resistance observed in this study indicates it will be difficult to use commonly use antibiotics to treat infection caused by S. typhimurium isolated in the study location

Date: 2024
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.rsisinternational.org/journals/ijrias/ ... -issue-8/211-222.pdf (application/pdf)
https://rsisinternational.org/journals/ijrias/arti ... ngs-in-nyanya-abuja/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:bjf:journl:v:9:y:2024:i:8:p:211-222

Access Statistics for this article

International Journal of Research and Innovation in Applied Science is currently edited by Dr. Renu Malsaria

More articles in International Journal of Research and Innovation in Applied Science from International Journal of Research and Innovation in Applied Science (IJRIAS)
Bibliographic data for series maintained by Dr. Renu Malsaria ().