A comparison between Hydrochloric acid and Trifluoroacetic acid in hydrolysis method of exopolysaccharide from Ophiocordyceps sinensis in Monosaccharide composition analysis by GC-FID

Le Thi Thuy Hang (), Ha Bao Chau, Tran Van Hai Nam, Pham Minh Thong, Dang Hoang Phu, Dinh Minh Hiep and Nguyen Tien Thang
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Le Thi Thuy Hang: Institute of Tropical Biology, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology; Faculty of Food Technology, Ho Chi Minh City University of Food Industry, Vietnam
Ha Bao Chau: Faculty of Biology & Biotechnology, VNUHCM-University of Science, Vietnam
Tran Van Hai Nam: Faculty of Biology & Biotechnology, VNUHCM-University of Science, Vietnam
Pham Minh Thong: Faculty of Biology & Biotechnology, VNUHCM-University of Science, Vietnam
Dang Hoang Phu: Department of Organic Chemistry, Faculty of Chemistry, VNUHCM-University of Science, Vietnam
Dinh Minh Hiep: Ho Chi Minh City Agricultural Hi-Tech Park, Ho Chi Minh City, Vietnam
Nguyen Tien Thang: Institute of Tropical Biology, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2020, vol. 10, issue 1, 29-36

Abstract: The monosaccharide composition is one of the crucial factors affecting the bioactivity of exopolysaccharide (EPS) in Cordyceps species. Therefore, many scientists have studied, analyzed monosaccharide composition and structure of EPS from Cordyceps species, especially Ophiocordyceps sinensis (O. sinensis). This study aimed to compare hydrochloric acid (HCl) with trifluoroacetic acid (TFA) in the EPS hydrolysis process in monosaccharide composition analysis by Gas Chromatography with Flame-Ionization Detection (GC-FID). The hydrolysis is a crucial step in forming the acetyl derivative, which helps the GC-FID technique to have good results in monosaccharide composition analysis. The results showed that hydrolysis with HCl gave a higher hydrolysis efficiency and was more suitable than hydrolysis by TFA in pretreatment to EPS for GC-FID. Hydrolysis results were analyzed through thin-layer chromatography and high-performance liquid chromatography (HPLC), then Acetyl derivatives were produced and finally analyzed by GC-FID to determine the monosaccharide composition of EPS. For EPS hydrolyzed by HCl, the analytical results presented that this sample had 6 kinds of monosaccharides, including rhamnose, arabinose, xylose, mannose, glucose, and galactose; the most monosaccharide was glucose. The EPS hydrolyzed by TFA only detected three kinds of monosaccharides, including mannose, arabinose, and galactose, mainly mannose. The study has set a foundation for further analysis of monosaccharide composition and structure of EPS from O. sinensis.

Keywords: EPS hydrolysis; GC-FID; HPLC; monosaccharide; O. sinensis (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:10:y:2020:i:1:p:29-36

DOI: 10.46223/HCMCOUJS.tech.en.10.1.357.2020

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