Isolation and selection of Bacillus strains with high potential probiotic that used in catfish farming (Pangasianodon hypophthalmus)

Nguyen Van Minh (), Nguyen Hoang Tuan Duy, Do Phuong Quynh, Tran Thi A Ni, Tran Kien Duc and Duong Nhat Linh
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Nguyen Van Minh: Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam
Nguyen Hoang Tuan Duy: Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam
Do Phuong Quynh: Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam
Tran Thi A Ni: MIDOLI Co., ltd, Vietnam
Tran Kien Duc: Ho Chi Minh City University of Science, Vietnam
Duong Nhat Linh: Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2020, vol. 10, issue 1, 3-16

Abstract: In this study, we isolated 28 strains of Bacillus spp. from water samples, catfish pond mud samples and earthworm manure (Perionyx excavates). By the cross-streak agar methods, 22 Bacillus strains showed the inhibition ability to Edwardsiella ictaluri, which caused Bacillary Necrosis Pangasius (BNP) in catfish (Pangasianodon hypophthalmus). Both Bacillus sp. Q16 and Q111 strains showed the highest inhibition to E. ictaluri by the double-layer agar methods. Finally, two Bacillus strains (Q16, Q111) were selected as a source of potential probiotic because of the ability of extracellular enzyme secretion (protease, amylase, cellulose) strong growth at 0,1-1% salt concentrations, survival within the pH range 6-8, resistance to low pH and low bile salts, inability to produce haemolysin enzyme, sensitivity to eight antibiotics in the three impacting groups (inhibition of wall synthesis, inhibition mechanism of protein synthesis, inhibition of nucleic acid synthesis). Two Bacillus strains (Q16, Q111) were identified that they belong to Bacillus subtilis by biochemical method and 16S rRNA gene sequencing method. This study indicated that two Bacillus strains (Q16, Q111) isolated from catfish pond can be applied as high potential probiotics that used to farm catfish.

Keywords: Bacillus subtilis; probiotic; Edwardsiella ictaluri; Perionyx excavatus; Pangasianodon hypophthalmus (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:10:y:2020:i:1:p:3-16

DOI: 10.46223/HCMCOUJS.tech.en.10.1.355.2020

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