Cytotoxic activity related to survivin mRNA levels by Combretum quadrangulare Kurz extract against liver and breast cancer cells

Nguyen Thi Phuong (), Duong Thuc Huy and Nguyen Huu Hung
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Nguyen Thi Phuong: NTT Hi-tech Institute, Nguyen Tat Thanh University, Vietnam
Duong Thuc Huy: Department of Chemistry, Ho Chi Minh City University of Education, Vietnam
Nguyen Huu Hung: Faculty of Technology, Van Lang University, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2022, vol. 12, issue 1, 55-64

Abstract: Liver cancer and breast cancer have high death rate and increased rapidly in recent years in Vietnam and over the world. Currently, the study of bioactive compounds isolated from plants for killing cancer cells is of interest. Herein, the study aims to identify the cytotoxicity using MTT assay of Combretum quadrangulare Kurz fractions against MCF-7 cells. RT-qPCR method was used to determine the survivin mRNA levels in P12 fraction-treated HepG2 and MCF-7 in comparison to DMSO-treated cells. The results showed that P12 fraction with IC50 of 20.9 ± 0.8 µg/mL was the best cytotoxicity on MCF-7 cells amongst P fractions. P12 fraction exhibited the apoptotic effect on HepG2 and MCF-7 cells by down-regulating survivin expression levels. Thus, P12 fraction was promising for the next research for the impact of fractions assessment in vivo or bioactive compounds isolation.

Keywords: apoptosis; breast cancer; Combretum quadrangulare; cytotoxicity; liver cancer; survivin (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:12:y:2022:i:1:p:55-64

DOI: 10.46223/HCMCOUJS.tech.en.12.1.1992.2022

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