Isolation and characterization of Rhizobium spp. and Bradyrhizobium spp. from legume nodules

Nguyen Thi Hanh Nguyen (), Vo Thi Minh Thao, Nguyen Minh Khanh, Pham Cam Duyen, Phung Thi Tram, Doan Thi Minh Thu, Le Thi Phuong Thao, Nguyen Ngoc Anh Vy, Ta Nguyen Minh Phuong, Tran Thi Kim Xinh, Nguyen Minh Quan and Nguyen Tan Duc
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Nguyen Thi Hanh Nguyen: Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Vo Thi Minh Thao: Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Nguyen Minh Khanh: Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Pham Cam Duyen: Ho Chi Minh City University of Technology (HCMUT), Vietnam National University
Phung Thi Tram: Ho Chi Minh City University of Technology (HCMUT), Vietnam National University
Doan Thi Minh Thu: Ton Duc Thang University, Ho Chi Minh City, Vietnam
Le Thi Phuong Thao: Ton Duc Thang University, Ho Chi Minh City, Vietnam
Nguyen Ngoc Anh Vy: Ton Duc Thang University, Ho Chi Minh City, Vietnam
Ta Nguyen Minh Phuong: Ho Chi Minh City University of Technology (HUTECH), Ho Chi Minh City, Vietnam
Tran Thi Kim Xinh: Ho Chi Minh University of Natural Resources and Environment, Ho Chi Minh City, Vietnam
Nguyen Minh Quan: Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Nguyen Tan Duc: Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2022, vol. 12, issue 2, 70-98

Abstract: Rhizobia topic has been re-focused in recent years because of new findings on their traits not only as nitrogen-fixing bacteria but also as plant growth-promoting rhizobacteria. When combing rhizobial strains with novel biological carriers (e.g., biochar) for inoculant production, it brings great potential for improving soil health in long-term. Appreciating this trend, this study is designed to isolate and characterize local rhizobial strains from legume fields using the conventional method with some modifications to increase efficiency in rhizobial identification. As a result, 17 rhizobial strains were isolated and classified biochemically that genetic identification outcome confirmed 10 strains belong to 07 different Rhizobium species as R. mayense, R. paknamense, R. pusense, R. miluonense, R. tropici, R. phaseoli, and R. multihospitium while the rest belong to 06 various Bradyrhizobium species as B. elkanii, B. centrosematis, B. guangxiense, B. liaoningense, B. yuanmingense, and B. arachidis. Thermal and saline tolerant tests together with seed germination tests also performed on these rhizobial strains to gain data on their responses to abiotic stresses. By comparing rice and mung bean GI values, we can assess the effectiveness of each rhizobial strains to help seeds at their early germination.

Keywords: Bradyrhizobium; germination; index nitrogen-fixing bacteria; legume nodule; Rhizobium (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:12:y:2022:i:2:p:70-98

DOI: 10.46223/HCMCOUJS.tech.en.12.2.2116.2022

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