Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics

Dao Thi Thanh Thuy, Phan Van Tien, Huy Van Duc, Nguyen Thi Ngoc Nhu, Phan Lu Chinh Nhan, Nguyen Ton Ngoc Huynh and Dang Thi Tung Loan ()
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Dao Thi Thanh Thuy: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam
Phan Van Tien: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam
Huy Van Duc: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam
Nguyen Thi Ngoc Nhu: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam
Phan Lu Chinh Nhan: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam
Nguyen Ton Ngoc Huynh: DNA International Hospital, Ho Chi Minh City, Vietnam
Dang Thi Tung Loan: University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2023, vol. 13, issue 2, 3-11

Abstract: Dermal Papilla Cells (DPCs) are mesenchymal cells residing at the base of the hair follicles. They play an important role in the hair cycle remodeling and hair follicle regeneration. In this study, DPCs were isolated, and cultured from human hair follicles and evaluated the expression of -SMA, versican by immunocytochemistry, and alkaline phosphatase activity. The human fibroblasts were used as a control to confirm the non-fibroblast DPCs. The results showed that the cells grew from the hair bulb and had fibroblast morphology. These bulb cells strongly expressed alkaline phosphatase activity -SMA, and versican while the human fibroblast lacked ALP activity and less expression of -SMA and versican. In conclusion, the protocol is established to isolate and culture DPCs from hair follicle bulbs, and ALP activity is suggested as the major marker for distinguishing DPCs and human fibroblasts.

Keywords: characteristic; dermal papilla; fibroblast; hair follicle; human (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:13:y:2023:i:2:p:3-11

DOI: 10.46223/HCMCOUJS.tech.en.13.2.2801.2023

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