Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

Bui Van Hoai (), Ngo Dai Nghiep, Dao An Quang and Nguyen Thi Nam Phuong
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Bui Van Hoai: University of Science, Viet Nam National University Ho Chi Minh City, Vietnam
Ngo Dai Nghiep: University of Science, Viet Nam National University Ho Chi Minh City, Vietnam
Dao An Quang: Ho Chi Minh City University of Food Industry, Vietnam
Nguyen Thi Nam Phuong: Ho Chi Minh City University of Food Industry, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2018, vol. 8, issue 1, 75-81

Abstract: Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

Keywords: antioxidant; chitooligosaccharide; DPPH; reducing power; UV-VIS (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:8:y:2018:i:1:p:75-81

DOI: 10.46223/HCMCOUJS.tech.en.8.1.339.2018

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