Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model

Tran Quoc Tuan, Dinh Thi Lan Anh, Le Thi Thuy Ai, Dinh Minh Hiep () and Tran Cat Dong
Additional contact information
Tran Quoc Tuan: University of Sciences,Viet Nam National University Ho Chi Minh City, Vietnam
Dinh Thi Lan Anh: University of Sciences,Viet Nam National University Ho Chi Minh City, Vietnam
Le Thi Thuy Ai: Agricultural Hi-Tech Park of Ho Chi Minh City, Vietnam
Dinh Minh Hiep: Agricultural Hi-Tech Park of Ho Chi Minh City, Vietnam
Tran Cat Dong: University of Medicine & Pharmacy at Ho Chi Minh City, Vietnam

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 2018, vol. 8, issue 1, 82-91

Abstract: Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in vivo. The result of the enzyme purification by membrane filtration method for enzyme recovery efficiency is 82.45%. Then the enzyme was purified to Q-Sepharose ion exchange chromatography with a purification factor of 1.82 and at a yield of 66.91%. Enzyme activity was 5,457 FU/mg protein. With the carrageenan-induced tail thrombosis in a rat model, results were significantly reduced by 67.3 to 83.6% at oral doses and injected nattokinase compared with the control group at 48 h. At 72 hours the rate increased to 82-89% in the oral dose and up to 91% in the injected dose nattokinase and comparable to 90% for heparin-positive hepatitis.

Keywords: bacillus subtilis; carrageenan; purification; recombinant nattokinase; thrombosis (search for similar items in EconPapers)
Date: 2018
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/907/758 (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:bjw:techen:v:8:y:2018:i:1:p:82-91

DOI: 10.46223/HCMCOUJS.tech.en.8.1.907.2018

Access Statistics for this article

HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY is currently edited by Nguyen Thuan

More articles in HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY from HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE, HO CHI MINH CITY OPEN UNIVERSITY
Bibliographic data for series maintained by Vu Tuan Truong ().