 Molecular detection of Brucella in milk using polymerase chain reactionA. Mohsen Czech Journal of Food Sciences, 2000, vol. 18, issue 3, 95-97 Abstract: Brucellosis is a highly contagious disease affecting a wide variety of farm animals. It is also an important zoonosis, and man is often infected following contact with infected animals or the consumption of contaminated milk and milk products. At present, mainly bacteriological and serological detection methods are used. A bacteriological method takes days to weeks to grow the organism besides its health hazard. Serological tests are faster but antigen-antibody interactions can be faulted by non-specific interactions. A method for direct detection of Brucella melitensis in 1 ml of milk was developed on the basis of enzymatic treatment of milk components and subsequent PCR and line probe assay (LPA). After PCR, 3 × 104 CFU/ml sensitivity was obtained by agarose gel electrophoresis and LPA. The safety and sensitivity of LPA combined with its speed suggests the potential of this technique for diagnosis of brucellosis in milk rather than the time consuming classical methods. Keywords: Brucella melitensis; milk; polymerase chain reaction (search for similar items in EconPapers) Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:18:y:2000:i:3:id:8318-cjfs DOI: 10.17221/8318-CJFS Access Statistics for this article Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová Ph.D. More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences |
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